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Bio-Rad
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Merck KGaA
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MD Biosciences
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Image Search Results
Journal: Journal of Tissue Engineering
Article Title: High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration
doi: 10.1177/2041731420919334
Figure Lengend Snippet: Immunohistochemical analysis of ASC collagen gel cultures after 14 days with 100 ng mL −1 rhGDF6 stimulation with and without SMAD1/5/8 (Dorso) or ERK1/2 (U0126) inhibition and no stimulation control. After 14 days culture, constructs stimulated with rhGDF6 demonstrated an increase in aggrecan and type II collagen deposition compared to rhGDF6 stimulated constructs where SMAD1/5/8 and ERK1/2 signalling had been blocked, where levels appeared similar to unstimulated controls. Scale bar, 500 µm.
Article Snippet: Sections were allowed to equilibrate to room temperature, formalin fixed and blocked prior to addition of either
Techniques: Immunohistochemical staining, Inhibition, Control, Construct